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Related Concept Videos

Metastasis02:30

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...

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Related Experiment Video

Updated: May 29, 2026

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients
08:25

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

Published on: September 9, 2020

High fragmentation characterizes tumour-derived circulating DNA.

Florent Mouliere1, Bruno Robert, Erika Arnau Peyrotte

  • 1SysDiag UMR3145-CNRS, National Centre of the Scientific Research/BIO-RAD, Montpellier, France.

Plos One
|September 13, 2011
PubMed
Summary
This summary is machine-generated.

Tumor-derived circulating DNA (ctDNA) fragmentation is higher in metastatic colorectal cancer patients. Optimal ctDNA quantification using Q-PCR is achieved with 60-100 bp fragments, correlating positively with tumor weight.

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Last Updated: May 29, 2026

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients
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Published on: September 9, 2020

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06:53

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Published on: June 8, 2019

Cell-Free DNA Integrity Analysis in Urine Samples
07:58

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Published on: January 5, 2017

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Circulating DNA (ctDNA) shows promise as a diagnostic tool for cancers like colorectal cancer (CRC).
  • Previous studies on ctDNA concentration and tumor-derived fragment proportion lack normalization, leading to discrepancies.
  • Standardizing ctDNA analysis is crucial for reliable diagnostic applications.

Purpose of the Study:

  • To rigorously investigate the size distribution of ctDNA.
  • To establish a reliable method for quantifying tumor-derived ctDNA fragments.
  • To correlate ctDNA characteristics with tumor burden and patient outcomes.

Main Methods:

  • Utilized a highly specific quantitative PCR (Q-PCR) assay to analyze ctDNA size distribution.
  • Employed athymic nude mice xenografted with human colon cancer cells (SW620, HT29) for controlled experiments.
  • Correlated findings with plasma samples from metastatic colorectal cancer (CRC) patients and healthy individuals.

Main Results:

  • Tumor-derived ctDNA fragmentation and concentration positively correlate with tumor weight.
  • Q-PCR quantification of ctDNA is optimal for fragments sized 60-100 bp.
  • Plasma samples from xenografted mice and CRC patients revealed specific ctDNA size profiles and significantly higher fragmentation in tumors.

Conclusions:

  • Metastatic CRC patients exhibit approximately 5-fold higher mean ctDNA fragmentation compared to healthy individuals.
  • ctDNA size distribution and fragmentation patterns offer valuable insights into tumor characteristics.
  • Optimized Q-PCR methods enhance the accuracy of ctDNA quantification for cancer diagnostics.