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Updated: May 29, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

A universal multiplex PCR strategy for 100-plex amplification using a hydrophobically patterned microarray.

Yang Li¹, Shu-Juan Guo, Ning Shao

¹Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, 200240, China.

|September 13, 2011

Summary

This summary is machine-generated.

A new universal multiplex PCR strategy enables over 100-plex amplification using a patterned microarray. This method simplifies complex DNA analysis for research and clinical diagnostics.

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Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

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Published on: March 13, 2018

Area of Science:

Molecular Biology
Biotechnology
Genomics

Background:

Simultaneous identification of multiple DNA sequences is crucial for research and clinical diagnostics.
Multiplex PCR is a key technique, but often requires extensive optimization due to primer interference.

Purpose of the Study:

To develop a universal multiplex PCR strategy for high-plex amplification.
To overcome the limitations of traditional multiplex PCR optimization.

Main Methods:

A novel microarray with hydrophilic microwells on a hydrophobic chip was designed.
Primer pairs tagged with universal sequences were physically separated in microwells.
PCR products were amplified from universal sequences for analysis.

Main Results:

Demonstrated feasibility with 11-plex detection of pneumonia pathogens.
Achieved a 116-plex PCR reaction with minimal optimization.
Validated effectiveness in detecting Duchenne Muscular Dystrophy gene deletions in clinical samples.

Conclusions:

The MPH&HPM strategy offers a general, simple, and flexible solution for high-plex nucleic acid analysis.
This method significantly reduces optimization time and effort.
Broad applications are expected in laboratory research and clinical diagnostics.