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DNA reorientation on Au nanoparticles: label-free detection of hybridization by surface enhanced Raman spectroscopy

Evanthia Papadopoulou1, Steven E J Bell

  • 1Innovative Molecular Materials Group, School of Chemistry and Chemical Engineering, Queen's University, Belfast, BT9 5AG, UK.

Chemical Communications (Cambridge, England)
|September 13, 2011
PubMed

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...