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Updated: May 29, 2026
Integrated Bone Formation Through In Vivo Endochondral Ossification Using Mesenchymal Stem Cells
Published on: July 14, 2023
[An experimental study of endothelial progenitor cells on bone formation in tissue-engineered bone in dogs]
Zhen-hua Gao1, Ling-xue Bu, Rong-tao Yuan
1Department of Oral and Maxillofacial Surgery, the Affiliated Hospital of Qingdao University Medical College. Qingdao 266003, Shandong Province, China. gaozhenhua2211@126.com
Purpose:
To observe the effects of endothelial progenitor cells (EPCs) on bone formation of tissue engineered bone in dogs.
Methods:
Bone marrow stromal cells (BMSCs) and EPCs were derived from dog bone marrow and cultured in different medium in vitro. They were seeded on demineralized bone matrix (DBM) to build tissue engineered bone, then the construct was implanted into the fasciae of latissimus dorsi muscle, the degree of bone formation was analyzed with imaging and histological methods at different time points. SPSS13.0 software package was used for statistical analysis.
Results:
4, 8, 12 weeks after operation, X-ray film showed bone mineral density (BMD) in the EPCs group was significantly higher than the control group, and there was significant difference between the two groups(P<0.05); Histological examination revealed that the degree of bone formation in the EPCs group was higher than the control group, the new bone area and blood vessel area between the two groups were significantly different(P<0.05).
Conclusion:
EPCs can promote bone formation and accelerate new bone formation in tissue-engineering bone.
