Related Experiment Video
Updated: May 29, 2026
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
Published on: March 8, 2012
[Effect of HPV 16 E5 on E6 and E7 gene induced human immortalized oral epithelial cell]
Xiao-xiao Hou1, Tong Jiang, Xin-quan Jiang
1Department of Stomatology, the Third Affiliated Hospital of Anhui Medical University. Hefei 230001, Anhui Province, China. hxx198402@sina.cn
Purpose:
To investigate the influence of HPV16 E5 on E6 and E7 gene in human oral immortalized epithelial cells (HIOEC).
Methods:
The pLEGFP-E5 was transferred into HIOEC cell; Deletion mutation expression vectors were constructed and transferred into HIOEC; RT-PCR was used to detect the gene expression in HIOEC; The expression of E6 and E7 mRNA was detected by real time-PCR;Cell proliferation of HIOEC was evaluated by MTT. All statistical analysis was performed by using SPSS 13.0 software package.
Results:
The deletion mutation expression vector of E5 was successfully constructed and applied for further experiments; E5 and the deletion mutation genes were transferred into HIOEC successfully; HPV16 E5 promoted cell proliferation of HIOEC and made the increased expression raised of E6 and E7 genes; However, the deletion mutation had no significant effect on HIOEC.
Conclusions:
HPV16 E5 is transferred into HIOEC successfully. E5 may promote cell proliferation and upregulate the mRNA expression of E6 and E7 genes.
