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Updated: May 29, 2026

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
Published on: August 26, 2009
Fluorescence microscopy of nanochannel-confined DNA
Fredrik Persson1, Fredrik Westerlund, Jonas O Tegenfeldt
1Department of Physics, University of Gothenburg, Gothenburg, Sweden.
Abstract:
Stretching of DNA in nanoscale confinement allows for direct visualization of the genetic contents of the DNA on the single DNA molecule level. DNA stretched in nanoscale confinement also allows for studies of DNA-protein interactions and DNA polymer physics in confined environments. This chapter describes the basic steps to fabricate the nanostructures, to perform the experiments, and to analyze the data.
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