Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Intracellular Signaling Affects Focal Adhesions

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...

Activation of Integrins

Activation of Integrins

Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.

Integrins

Integrins

Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An Insight Into a Potential Antithrombin-Independent Mechanism of Inadequate Heparin Response in Neonates and Infants Undergoing Pediatric Cardiac Surgery: An Exploratory Study.

Journal of cardiothoracic and vascular anesthesia·2026

Same author

Postoperative pulmonary complications after neuromuscular blockade: what does the recent evidence demonstrate?

Journal of thoracic disease·2026

Same author

Risk Factor Analysis of Inadequate Heparin Response in Pediatric Cardiac Surgery.

Journal of cardiothoracic and vascular anesthesia·2026

Same author

Plasma Proteomic Signatures of Pediatric Sepsis Reveal Persistent Inflammation and Phase-Specific Biomarkers.

FASEB bioAdvances·2026

Same author

Damage-Associated Molecular Patterns in Perioperative Anesthesia Care: A Clinical Perspective.

Anesthesia research·2026

Same author

Single-cell analysis of B cell dysregulation in pediatric sepsis stratified by disease severity.

Scientific reports·2025

Search research articles

Related Experiment Video

Updated: May 29, 2026

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

Cell-free ligand-binding assays for integrin LFA-1.

¹Department of Anesthesiology, Perioperative and Pain Medicine, Children's Hospital Boston, Boston, MA, USA. yuki@idi.harvard.edu

Methods in Molecular Biology (Clifton, N.J.)

|September 13, 2011

Summary

This study details cell-free assays for examining interactions between Integrin LFA-1 and ICAM-1. These methods aid in discovering new drugs to block these interactions and treat inflammatory conditions.

Area of Science:

Immunology
Cell Biology
Drug Discovery

Background:

Leukocyte trafficking and T cell interactions are crucial in inflammation and autoimmune diseases.
Integrin LFA-1 mediates these processes by binding to ICAM-1.
Targeting LFA-1:ICAM-1 interactions offers a therapeutic strategy for inflammatory disorders.

Purpose of the Study:

To describe cell-free assay methodologies for studying LFA-1:ICAM-1 interactions.
To provide tools for screening potential LFA-1 antagonists.

Main Methods:

Development and description of cell-free assay systems.
Utilizing these assays to analyze LFA-1:ICAM-1 binding.

Main Results:

Established functional cell-free assays for LFA-1:ICAM-1 interaction studies.

More Related Videos

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

Published on: June 29, 2016

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs

Published on: February 2, 2024

Related Experiment Videos

Last Updated: May 29, 2026

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

Published on: June 29, 2016

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs

Published on: February 2, 2024

Demonstrated the utility of these assays for screening LFA-1 antagonists.

Conclusions:

Cell-free assays provide a valuable platform for investigating LFA-1:ICAM-1 interactions.
These assays can accelerate the discovery of novel therapeutics for inflammatory and autoimmune diseases.