Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Epithelial polymeric immunoglobulin receptors.

J C Huff1

  • 1Department of Dermatology, University of Colorado, Denver 80262.

The Journal of Investigative Dermatology
|June 1, 1990
PubMed
Summary

Secretory component (SC) was detected in normal human skin, particularly along the basement membrane zone and on epidermal cells. Cultured keratinocytes showed some SC expression, suggesting a potential role in skin

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Examination of the oral mucosa and peripheral blood cells of patients with recurrent aphthous ulceration for human herpesvirus DNA.

Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics·2000
Same author

Elevated levels of interferon gamma, tumor necrosis factor alpha, interleukins 2, 4, and 5, but not interleukin 10, are present in recurrent aphthous stomatitis.

Archives of dermatology·1998
Same author

A novel childhood pemphigus vegetans variant of intraepidermal neutrophilic IgA dermatosis.

Journal of the American Academy of Dermatology·1998
Same author

Misdiagnosis, treatments, and outcomes in the immunobullous diseases in children.

Pediatric dermatology·1997
Same author

Intraepidermal neutrophilic IgA dermatosis in a patient with advanced human immunodeficiency virus infection.

Archives of dermatology·1997
Same author

Lesion size is a factor for determining the rate of port-wine stain clearing following pulsed dye laser treatment in adults.

Cutis·1997

Area of Science:

  • Immunology
  • Dermatology
  • Cell Biology

Background:

  • The secretory immune system's role in skin immunity is unclear.
  • Secretory component (SC), a key protein in this system, has been observed in skin structures like sweat glands.

Purpose of the Study:

  • To investigate the presence and location of SC in normal human skin and cultured keratinocytes.
  • To explore the potential function of SC in cutaneous immunology.

Main Methods:

  • Immunohistochemistry using polyclonal and monoclonal antibodies against SC on normal human skin.
  • Indirect immunofluorescence (IF) staining of cultured human keratinocytes for SC.
  • Immunoblotting analysis of cell lysates from keratinocytes and control cells (HT 29).

Main Results:

  • SC was positively stained along the basement membrane zone of normal human skin.
  • Focal SC staining was observed on the surfaces of epidermal cells.
  • Cultured human keratinocytes exhibited granular cell-surface fluorescence for SC, albeit at lower intensity than controls.
  • Immunoblotting of cell lysates was negative, possibly due to protein degradation or loss of antigenicity.

Conclusions:

  • Human keratinocytes may express SC, potentially contributing to skin's defense against pathogens.
  • SC expression in skin could be relevant to IgA deposition observed in certain dermatological conditions.

Related Experiment Videos