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Related Concept Videos

Viral Replication: Lysogenic Cycle01:16

Viral Replication: Lysogenic Cycle

The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects its...
DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the lytic replication...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Viral Replication: Lytic Cycle

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Updated: May 29, 2026

Following Cell-fate in E. coli After Infection by Phage Lambda
06:10

Following Cell-fate in E. coli After Infection by Phage Lambda

Published on: October 14, 2011

Bacteriophage recombineering in the lytic state using the lambda red recombinases.

Tamás Fehér1, Ildikó Karcagi, Frederick R Blattner

  • 1Institute of Biochemistry, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary. fehert@brc.hu

Microbial Biotechnology
|September 14, 2011
PubMed
Summary

Bacteriophage recombineering with electroporated DNA (BRED) successfully engineered a coliphage, P1virdeltaIS, by removing an active mobile element. This IS-free phage functions normally, offering a valuable tool for bacteriophage genome engineering.

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Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
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Published on: January 8, 2015

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Last Updated: May 29, 2026

Following Cell-fate in E. coli After Infection by Phage Lambda
06:10

Following Cell-fate in E. coli After Infection by Phage Lambda

Published on: October 14, 2011

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

Area of Science:

  • Molecular biology
  • Microbiology
  • Biotechnology

Background:

  • Bacteriophages are crucial model organisms in molecular biology.
  • Developing efficient genetic engineering techniques for bacteriophages is essential for biotechnological applications.

Purpose of the Study:

  • To report the first use of bacteriophage recombineering with electroporated DNA (BRED) in a coliphage.
  • To engineer a coliphage genome by removing an active mobile element (IS1).

Main Methods:

  • Bacteriophage recombineering with electroporated DNA (BRED) was employed.
  • A copy of the mobile element IS1 was removed from the P1vir coliphage genome.

Main Results:

  • The engineered IS-free coliphage, P1virdeltaIS, exhibited normal plaque morphology, phage titre, burst size, and generalized transduction capabilities.
  • Competition experiments showed P1vir could not outperform P1virdeltaIS, indicating IS1 is not essential for lytic replication.

Conclusions:

  • P1virdeltaIS serves as a genome engineering vehicle free from IS contamination.
  • BRED is a versatile tool applicable for engineering bacteriophage genomes across various taxa.