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Updated: May 29, 2026

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Published on: October 7, 2020
Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001
Jibin Zhang1, Pengxia Wu, Bo Hao
1State Key Laboratory of Agricultural Microbiology and National Engineering Research Centre of Microbial Pesticides, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Abstract:
A strain YZN-001 was isolated from swine manure effluent and was identified as Pseudomonas stutzeri. It can utilise not only nitrate and nitrite, but also ammonium. The strain had the capability to fully remove as much as 275.08 mg L(-1) NO(3)(-)-N and 171.40 mg L(-1) NO(2)(-)-N under aerobic conditions. Furthermore, At 30°C, the utilization of ammonium is approximately 95% by 18 h with a similar level removed by 72 h and 2 weeks at 10 and 4°C, respectively. Triplicate sets of tightly sealed serum bottles were used to test the heterotrophic nitrifying ability of P. stutzeri YZN-001. The results showing that 39% of removed NH(4)(+)-N was completely oxidised to nitrogen gas by 18 h. Indicating that the strain has heterotrophic nitrification and aerobic denitrification abilities, with the notable ability to remove ammonium at low temperatures, demonstrating a potential using the strain for future application in waste water treatment.
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