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Published on: August 21, 2021
Photodynamically crosslinked and chitosan-incorporated dentin collagen.
A Shrestha1, S Friedman, A Kishen
1Discipline of Endodontics, Faculty of Dentistry, University of Toronto, 124 Edward Street, Toronto, ON M5G1G6, Canada.
Journal of Dental Research
|September 14, 2011
Summary
Improving dentin stability after root canal treatment is crucial. This study shows that crosslinking dentin collagen with carboxymethyl-chitosan (CMCS) enhances its resistance to degradation and improves mechanical strength, offering better long-term outcomes for restored teeth.
Area of Science:
- Biomaterials Science
- Dental Materials
- Biochemistry
Background:
- Restored root-filled teeth often suffer from compromised dentin structural integrity.
- The dentin-sealer interface is susceptible to degradation over time, impacting restoration longevity.
Purpose of the Study:
- To investigate the impact of combined chemical/photodynamic crosslinking and carboxymethyl-chitosan (CMCS) incorporation on dentin collagen.
- To evaluate the effects on enzymatic degradation resistance and mechanical properties of dentin collagen.
Main Methods:
- Preparation of demineralized dentin collagen specimens (human and bovine).
- Application of chemical crosslinking (glutaraldehyde, carbodiimides) and photodynamic crosslinking (rose Bengal, 540 nm light).
- Incorporation of carboxymethyl-chitosan (CMCS) into the collagen matrix.
- Assessment via enzymatic degradation tests, transmission electron microscopy (TEM), and tensile testing.
Main Results:
- Crosslinked dentin collagen exhibited significantly enhanced resistance to enzymatic degradation (p < 0.01).
- Ultrastructural stability was maintained, and tensile strength increased significantly (p < 0.05).
- CMCS incorporation further improved mechanical properties (p < 0.01) through crosslinking with the collagen matrix.
Conclusions:
- Combined chemical/photodynamic crosslinking of dentin collagen with CMCS offers a promising strategy.
- This approach significantly improves dentin collagen's resistance to degradation and enhances its toughness.
- The findings suggest a potential method to improve the durability of restored root-filled teeth.
