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Fluorescence detection by intensity change based sensors: a theoretical model.

Javier Galbán¹, Arantzazu Delgado-Camón, Vicente L Cebolla

¹Analytical Biosensors Group, Institute of Nanoscience of Aragón, Analytical Chemistry Department, Faculty of Science, University of Zaragoza, Zaragoza, 50009, Spain. jgalban@unizar.es

Journal of Fluorescence

|September 14, 2011

Summary

This study introduces Fluorescence Detection by Intensity Changes (FDIC) using sensor films. Immobilized fluorophores in polyacrylamide films offer a novel analytical method, with film volume changes being a key detection mechanism.

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Area of Science:

Analytical Chemistry
Materials Science
Biophysics

Background:

Fluorescence intensity of fluorophores is influenced by molecular interactions.
Fluorescence Detection by Intensity Changes (FDIC) leverages these interactions for analytical purposes.
Utilizing fluorophores in solid-state sensor films offers potential for novel detection methods.

Purpose of the Study:

To develop and validate a mathematical model for FDIC using immobilized fluorophores in sensor films.
To investigate the application of sensor films for determining analyte concentrations.
To compare the performance of sensor films with solution-based FDIC.

Main Methods:

Development of a mathematical model linking analyte concentration to immobilized fluorophore fluorescence.
Experimental validation using various fluorophores and solid supports, focusing on polyacrylamide (PAA) and coralyne.
Application of the sensor film for albumin and polyethylenglycol determination.

Main Results:

The best sensor film performance was achieved with polyacrylamide (PAA) polymers and coralyne.
Albumin quenched coralyne fluorescence in both solution and film formats.
Polyethylenglycol (PEG) showed differential effects: quenching in films but enhancement in solution, suggesting film volume changes as a primary mechanism.

Conclusions:

Sensor films provide a viable platform for FDIC applications.
The dominant fluorescence change mechanism in sensor films appears to be alterations in film volume, differing from solution-based interactions.
This approach offers a new avenue for quantitative analysis of analytes like albumin and PEG.