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Related Concept Videos

Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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Related Experiment Video

Updated: May 29, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

DNA methylation detection: bisulfite genomic sequencing analysis.

Yuanyuan Li1, Trygve O Tollefsbol

  • 1Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 14, 2011
PubMed
Summary

DNA methylation is crucial for gene expression and development. This study details a reliable bisulfite sequencing protocol for analyzing DNA methylation patterns, aiding in disease diagnosis and treatment.

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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

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Last Updated: May 29, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

Area of Science:

  • Molecular Biology
  • Epigenetics

Background:

  • DNA methylation, primarily at CpG sites, regulates critical biological processes including gene expression, development, and cellular stability.
  • Dysregulation of DNA methylation is linked to genomic instability and diseases like cancer, necessitating precise analytical methods.
  • Bisulfite genomic sequencing is a foundational technique for DNA methylation analysis, offering high sensitivity and quantitative insights.

Purpose of the Study:

  • To present a refined bisulfite sequencing protocol for accurate DNA methylation analysis.
  • To provide practical guidance on troubleshooting common technical issues in methylation studies.

Main Methods:

  • Utilizes sodium bisulfite conversion of genomic DNA.
  • Employs bisulfite genomic sequencing for qualitative and quantitative DNA methylation profiling.
  • Presents a laboratory-tested protocol for optimal results.

Main Results:

  • The described protocol yields optimal outcomes for DNA methylation analysis.
  • The method is adaptable for various applications in epigenetics research.
  • Troubleshooting notes are provided for common technical challenges.

Conclusions:

  • The presented bisulfite sequencing protocol is a valuable tool for studying DNA methylation.
  • Accurate DNA methylation analysis is essential for understanding disease mechanisms and developing therapies.
  • This protocol facilitates further research into the role of epigenetics in health and disease.