Related Experiment Video
Updated: May 29, 2026

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
Published on: March 31, 2019
Restriction landmark genome scanning
Hisato Okuizumi1, Tomoko Takamiya, Yasushi Okazaki
1Genetic Resources Center, National Institute of Agrobiological Sciences (NIAS), Tsukuba, Ibaraki, Japan. okuizumi@affrc.go.jp
This study presents an improved Restriction Landmark Genome Scanning (RLGS) method for precise genome-wide methylation analysis. The enhanced technique simplifies epigenetic research and aids in discovering novel epigenetic phenomena in plants and animals.
Area of Science:
- Epigenetics
- Genomics
- Molecular Biology
Background:
- Restriction Landmark Genome Scanning (RLGS) is a high-resolution method for analyzing whole genome DNA, including methylation status.
- Conventional RLGS detects both polymorphism and methylated NotI sites, with applications in gene cloning and cancer research.
Purpose of the Study:
- To develop an improved RLGS method for specifically detecting methylated sites using isoschizomer restriction enzymes.
- To enhance the efficiency of RLGS spot site identification using genome database information.
- To map genome methylation sites and investigate epigenetic phenomena in plants and animals.
Main Methods:
- Developed an improved RLGS method utilizing isoschizomer restriction enzymes (MspI and HpaII) for differential sensitivity to methylated sequences.
- Employed genome database information for efficient identification of RLGS spot sites.
- Applied the improved method to map Arabidopsis thaliana genome methylation and analyze rice gene methylation inheritance.
Main Results:
- Successfully mapped genome methylation sites in Arabidopsis.
- Detected unique inheritance patterns in methylated genes in rice.
- Demonstrated the improved RLGS method's efficiency in identifying methylation sites and facilitating epigenetic research.
Conclusions:
- The improved RLGS method offers a more specific and efficient approach to genome-wide methylation analysis.
- This technique simplifies epigenetic research and has broad applicability in both plant and animal genomes.
- The RLGS method is valuable for exploring novel epigenetic phenomena and understanding gene regulation.
More Related Videos
09:06High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
Published on: October 5, 2018
08:54In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
Published on: March 29, 2019
Related Concept Videos
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Homologous Recombination
Genome-wide Association Studies-GWAS
GWAS does not require the identification of the target gene involved in...