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Related Concept Videos

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

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Updated: May 29, 2026

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
14:29

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Published on: May 1, 2013

A chromatin immunoprecipitation protocol for small cell numbers.

Philippe Collas1

  • 1University of Oslo, Institute of Basic Medical Sciences & Norwegian Center for Stem Cell Research, Oslo, Norway. philippe.collas@medisin.uio.no

Methods in Molecular Biology (Clifton, N.J.)
|September 14, 2011
PubMed
Summary

Micro (μ)ChIP enables multiple protein-DNA interaction analyses from just 1,000 cells. This chromatin immunoprecipitation method expands ChIP applications for rare or small cell samples.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Epigenetics

Background:

  • Chromatin immunoprecipitation (ChIP) analyzes protein-DNA interactions.
  • Conventional ChIP requires large cell numbers, limiting rare sample analysis.
  • Recent advances allow ChIP with 10,000-100,000 cells.

Purpose of the Study:

  • To describe a micro (μ)ChIP procedure.
  • To enable multiple parallel ChIPs from a single chromatin batch.
  • To utilize minimal cell input (1,000 cells).

Main Methods:

  • Development of a micro (μ)ChIP protocol.
  • Parallel ChIP assays.
  • Utilizing a single chromatin batch from 1,000 cells.

Main Results:

  • Successful implementation of micro (μ)ChIP.
  • Demonstrated feasibility of multiple parallel ChIPs.
  • Enabled ChIP analysis from significantly reduced cell input.

Conclusions:

  • Micro (μ)ChIP is a powerful technique for protein-DNA interaction studies.
  • This method significantly lowers the cell number requirement for ChIP.
  • Expands ChIP applicability to rare and small cell populations.