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Related Concept Videos

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

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Related Experiment Video

Updated: May 29, 2026

Chromatin Immunoprecipitation of Murine Brown Adipose Tissue
07:50

Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

Published on: November 21, 2018

Native chromatin immunoprecipitation.

Céline Cosseau1, Christoph Grunau

  • 1Ecologie et Evolution des Interactions, 2EI, Université de Perpignan, Perpignan Cedex, France.

Methods in Molecular Biology (Clifton, N.J.)
|September 14, 2011
PubMed
Summary
This summary is machine-generated.

Native chromatin immunoprecipitation (NChIP) identifies DNA linked to proteins without structural changes. This optimized protocol successfully works with low cell numbers, offering an alternative to cross-linking methods.

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Last Updated: May 29, 2026

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Area of Science:

  • Molecular Biology
  • Genomics
  • Epigenetics

Background:

  • Native chromatin immunoprecipitation (NChIP) is a technique for studying DNA-protein interactions.
  • NChIP preserves native protein structure, unlike cross-linking methods.
  • Existing NChIP protocols often require large cell numbers.

Purpose of the Study:

  • To present an optimized protocol for native chromatin immunoprecipitation.
  • To enable NChIP analysis using significantly reduced cell input.
  • To provide a valuable alternative to cross-linking chromatin immunoprecipitation.

Main Methods:

  • Development of a protocol for native chromatin immunoprecipitation.
  • Optimization of the protocol for low cell number input.
  • Validation of the protocol for DNA-protein association studies.

Main Results:

  • Successful implementation of NChIP with limited cell samples.
  • Demonstration of the protocol's efficacy in identifying DNA associated with chromatin proteins.
  • Preservation of native protein structure throughout the procedure.

Conclusions:

  • The optimized NChIP protocol is effective for low cell numbers.
  • This method offers advantages over cross-linking techniques for specific applications.
  • The protocol facilitates the study of chromatin structure and DNA interactions in samples with limited biological material.