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Updated: May 29, 2026
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Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells
Published on: April 2, 2018
Mapping open chromatin with formaldehyde-assisted isolation of regulatory elements
Takao Nammo1, Santiago A Rodríguez-Seguí, Jorge Ferrer
1Genomic Programming of Beta Cells, Institut d'Investigacions Biomediques August Pi i Sunyer, Barcelona, Spain.
Methods in Molecular Biology (Clifton, N.J.)
|September 14, 2011
Summary
Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) identifies active genomic regions in cells. This study details implementing FAIRE in human pancreatic islet cells to better understand gene regulation.
Area of Science:
- Genomics
- Epigenetics
- Cellular Biology
Background:
- Noncoding genomic elements regulate crucial cellular processes, including differentiation and disease.
- These regulatory elements are not well understood.
- Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) is a method to identify active regulatory sequences based on nucleosomal density.
Purpose of the Study:
- To implement the FAIRE method in human pancreatic islet cells.
- To enable genome-wide identification of regulatory elements in pancreatic islets.
Main Methods:
- Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) protocol.
- Application of FAIRE to human pancreatic islet cells.
- Analysis of regulatory element characteristics.
Main Results:
- Successful implementation of the FAIRE method in human pancreatic islet cells.
- Identification of active regulatory genomic elements within these cells.
- Provides a foundation for further studies on pancreatic islet gene regulation.
Conclusions:
- FAIRE is a viable method for studying regulatory elements in human pancreatic islets.
- This technique facilitates the genome-scale characterization of tissue-specific regulatory sequences.
- Enables deeper insights into the genetic basis of pancreatic islet function and disease.
