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New Bapx1(Cre-EGFP) mouse lines for lineage tracing and conditional knockout studies
V Sivakamasundari1, Hsiao Yun Chan, Sook Peng Yap
1Stem Cell and Developmental Biology, Genome Institute of Singapore, Singapore 138672.
Genesis (New York, N.Y. : 2000)
|September 14, 2011
Summary
We developed two new Bapx1 mouse lines for studying gene function. These lines enable lineage tracing and conditional knockout of Bapx1 (Bapx1-Cre-EGFP) to understand its role in development.
Area of Science:
- Developmental Biology
- Genetics
- Molecular Biology
Background:
- Genetic studies are crucial for understanding gene function in development.
- Embryonic lethality often complicates genetic research in mice.
- Cre-lox technology and lineage tracing are vital tools in developmental studies.
Purpose of the Study:
- To create novel mouse models for studying the Bapx1 gene.
- To enable lineage tracing of Bapx1-expressing cells.
- To facilitate conditional knockout of Bapx1 for functional analysis.
Main Methods:
- Utilized internal ribosome entry site (IRES) and 2A peptide multicistronic expression strategies.
- Generated two new Bapx1(Cre-EGFP) mouse lines.
- Crossed Bapx1(Cre-EGFP) mice with Rosa26R-lacZ reporter mice.
Main Results:
- Developed Bapx1 mouse lines expressing Cre and enhanced green fluorescence protein (EGFP) under the Bapx1 promoter.
- Demonstrated utility for lineage tracing of Bapx1-expressing cells using the reporter line.
- Showcased EGFP fluorescence for visualizing stage-specific and spatial Bapx1 expression.
Conclusions:
- The new Bapx1(Cre-EGFP) mouse lines are effective tools for lineage tracing.
- These lines facilitate conditional knockout of Bapx1 to investigate gene function.
- The models aid in studying Bapx1's role in skeletal and visceral organ development.
