Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Vitamins in Maintaining Bone Health01:25

Role of Vitamins in Maintaining Bone Health

The growth and maintenance of bone are regulated by a combination of nutritional factors, including vitamins, such as vitamin A, B12, C, D, and K.
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
Sulfur Assimilation01:20

Sulfur Assimilation

Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to become...
Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...
Essential Minerals for Bone Health01:31

Essential Minerals for Bone Health

The minerals contained in all of the food we consume are essential for our organ systems. However, certain essential minerals, such as calcium, phosphorus, magnesium, manganese, and fluoride, largely affect bone health.
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lysophosphatidylcholine inhibits bradykinin-induced phosphoinositide hydrolysis and calcium transients in cultured bovine aortic endothelial cells.

Circulation research·1992
Same author

Retrovirus-mediated gene transfer targeted to malignant glioma cells in murine brain.

Japanese journal of cancer research : Gann·1992
Same author

Enhancement of DNA synthesis in rat thymocytes by stimulating their muscarinic acetylcholine receptors.

Cellular and molecular biology (Noisy-le-Grand, France)·1992
Same author

Different posttranslational processing of human preprothyrotropin-releasing hormone in the human placenta and hypothalamus.

The Journal of clinical endocrinology and metabolism·1992
Same author

The rat neurotensin receptor expressed in Chinese hamster ovary cells mediates the release of inositol phosphates.

Journal of neurochemistry·1992
Same author

New technique using intraductal ultrasonography for the diagnosis of diseases of the pancreatobiliary system.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine·1992

Related Experiment Videos

N-acetyl cysteine protects osteoblastic function from oxidative stress.

T Ueno1, M Yamada, Y Igarashi

  • 1Laboratory for Bone and Implant Sciences-LBIS, The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry, Los Angeles, California, USA. t.ueno.rpro@tmd.ac.jp

Journal of Biomedical Materials Research. Part A
|September 14, 2011
PubMed
Summary

N-acetyl cysteine (NAC) protects osteoblasts from oxidative stress by restoring cell proliferation, differentiation, and mineralization. NAC improves redox balance, showing therapeutic potential for bone health.

Related Experiment Videos

Area of Science:

  • Biochemistry
  • Cell Biology
  • Biomedical Engineering

Background:

  • Oxidative stress significantly impairs osteoblast function.
  • N-acetyl cysteine (NAC) is an antioxidant with potential therapeutic applications.

Purpose of the Study:

  • To investigate the protective effects of NAC against hydrogen peroxide (H₂O₂)-induced oxidative stress in osteoblasts.
  • To evaluate NAC's impact on osteoblast proliferation, differentiation, and mineralization.

Main Methods:

  • Osteoblastic cells were cultured and exposed to H₂O₂ to induce oxidative stress.
  • Co-treatment with varying concentrations of NAC was performed.
  • Cell proliferation, gene expression (collagen type I, osteopontin, osteocalcin), and mineralization were assessed.

Main Results:

  • H₂O₂ reduced cell number by 50%; NAC co-treatment dose-dependently restored cell numbers.
  • H₂O₂ downregulated key bone formation genes; NAC fully recovered their expression.
  • NAC co-treatment significantly enhanced mineral deposition compared to H₂O₂ alone.
  • NAC increased intracellular glutathione and decreased reactive oxygen species.

Conclusions:

  • Oxidative stress severely damages osteoblast proliferation, differentiation, and mineralization.
  • NAC effectively restores these functions by improving the cellular redox balance.
  • NAC demonstrates significant therapeutic potential as a local antioxidant for bone conditions.