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[Study of three ciguatera fish poisoning cases in Xiamen city, in 2005]
He-dong Luo1, Yan-yan Bai, Na Zhou
1Department of Detection, Xiamen Center for Disease Control and Prevention, Xiamen 361021, China. luohedong@126.com
Objective:
To find out the reason of three ciguatera fish poisoning cases in Xiamen in 2005 and identify the fish species.
Methods:
The grouper implicated in food poisoning and seven other coral reef fishes collected from market were tested by mice bioassay and ciguatoxin-test kit. The mtDNA was extracted from toxic grouper meat, and Cty b gene segment was amplified and the PCR products were sequenced. The sequences were compared with those in the GenBank.
Results:
The result turned out to be positive by the ciguatoxin-test kit, while the toxicity of the toxic grouper implicated in food poisoning was 0.11 mouse unit (MU)/g by mice bioassay. A 475 bp segments of Cty b gene was amplified by PCR and the sequence was 99% homologous with Epinephelus fuscoguttatus (GenBank: AY950695).No ciguatoxin in six grouper species collected from market was detected.
Conclusion:
All three food poisoning cases were caused by consumption of ciguatoxin-carrying groupers.
