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Updated: May 29, 2026
Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization
Published on: September 1, 2018
[Effects of PRL-3 siRNA on the migration of endometriotic stromal cells]
Fei Ruan1, Rui-jin Wu, Kai-hong Xu
1Department of Obstetrics & Gynecology, Zhejiang University, Hangzhou 310006, China.
Objective:
To evaluate the effects of PRL-3 siRNA (small interfering RNA) on the migration of endometriotic stromal cells.
Methods:
Primary endometriotic stromal cells were cultured in vitro. Then PRL-3 (phosphatase of regenerating liver-3) siRNA was transfected to silence the PRL-3 gene. And the PRL-3 protein expression was analyzed by Western blot. The changes of cell migration were detected by cell pseudopod formation, scratch test and transwell cell migration test.
Results:
The expression of PRL-3 protein significantly decreased in the experimental group versus two other control groups (P < 0.05). The formation of cell pseudopod was much less in experimental group than that in control groups. Within the same time, the number of migration cells was 0.87 ± 0.21 in experimental group. It was less than 1.75 ± 0.28 in blank control group and 1.63 ± 0.39 in negative control group (P < 0.05).
Conclusion:
PRL-3 siRNA can down-regulate the PRL-3 gene and decrease the migratory capacity of endometriotic stromal cells. And PRL-3 may be a promising target in the therapeutics of endometriosis.
Insights
Small interfering RNA (siRNA) targeting the PRL-3 gene significantly reduced the migration of endometriotic stromal cells. This suggests PRL-3 is a potential therapeutic target for endometriosis treatment.
Area of Science:
- Cell Biology
- Molecular Medicine
- Gynecology
