Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hypoxia01:23

Hypoxia

Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Lipid Catabolism01:25

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
Acute Respiratory Failure-II01:21

Acute Respiratory Failure-II

Type I Respiratory Failure, or hypoxemic respiratory failure, occurs when the partial pressure of oxygen (PaO2) in arterial blood falls below 60 mmHg while breathing room air without a corresponding increase in arterial carbon dioxide levels (PaCO2). This condition highlights a significant impairment in the lungs' capacity to oxygenate the blood.
The underlying physiological abnormalities that contribute to hypoxemic respiratory failure include:
Lipid-derived Compounds in the Human Body01:31

Lipid-derived Compounds in the Human Body

Fats and lipids are crucial components in the human body. Some lipid-derived compounds, such as fat-soluble vitamins, eicosanoids, lipoproteins, and glycolipids, also play unique roles to support various  biological processes .
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin, delayed...
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vascular-related proteomic signatures in COPD with suspected pulmonary hypertension as predictors of FEV₁ impairment.

Respiratory research·2026
Same author

Cardiomyocyte NLRP3 signaling in right heart failure is sexually dimorphic via estrogen receptor α.

bioRxiv : the preprint server for biology·2026
Same author

Sex-Based Differences in Cell Types and Gene Expression within the Anterior Cruciate Ligament.

The Journal of bone and joint surgery. American volume·2026
Same author

Sexually dimorphic role of estrogen receptor α in preserving right ventricular endothelial integrity.

bioRxiv : the preprint server for biology·2025
Same author

3D genetic architecture of schizophrenia risk across three neuronal subtypes.

Molecular psychiatry·2025
Same author

Early Sport Specialization and Intense Training in Junior Tennis Players: A Sport-Specific Review.

Sports health·2025
Same journal

Correction: Characterization of Mast2 kinase defines structural features, regulation, and substrates.

The Journal of biological chemistry·2026
Same journal

Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

The Journal of biological chemistry·2026
Same journal

Introduction to the Thematic Review Series on Intracellular Protein Degradation. The ubiquitous biology of intracellular protein degradation: a tribute to Alfred L. ("Fred") Goldberg.

The Journal of biological chemistry·2026
Same journal

Correction: Aromatic residue-rich amino-terminal segments of temporin L self-assemble into collagen-mimetic peptides with cell-adhesion properties.

The Journal of biological chemistry·2026
Same journal

YhbO is a DJ-1 family glyoxalase and α-oxoaldehyde hydratase that confers resistance to reactive carbonyl stress (112).

The Journal of biological chemistry·2026
Same journal

ARMH3 acts as a central scaffold at the Golgi/TGN through interactions with Arl5, GBF1, and PI4KB.

The Journal of biological chemistry·2026
See all related articles

Related Experiment Video

Updated: May 29, 2026

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

Dihydroceramide-based response to hypoxia.

Cecilia M Devlin1, Tim Lahm2, Walter C Hubbard3

  • 1Department of Medicine, Indiana University, Indianapolis, Indiana 46202.

The Journal of Biological Chemistry
|September 15, 2011
PubMed
Summary
This summary is machine-generated.

Hypoxia rapidly increases dihydroceramides (DHCs) in cells and tissues, independent of hypoxia-inducible factors. This DHC increase, catalyzed by dihydroceramide desaturase (DEGS), acts as an oxygen sensor regulating ceramide metabolism.

More Related Videos

Tracking Hypoxic Signaling within Encapsulated Cell Aggregates
09:14

Tracking Hypoxic Signaling within Encapsulated Cell Aggregates

Published on: December 16, 2011

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

Related Experiment Videos

Last Updated: May 29, 2026

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

Tracking Hypoxic Signaling within Encapsulated Cell Aggregates
09:14

Tracking Hypoxic Signaling within Encapsulated Cell Aggregates

Published on: December 16, 2011

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

Area of Science:

  • Biochemistry
  • Cell Biology
  • Physiology

Background:

  • Cellular responses to hypoxia are critical for survival.
  • Ceramide metabolism plays a role in cellular signaling.
  • The precise mechanisms of ceramide-based responses to hypoxia remain unclear.

Purpose of the Study:

  • To elucidate the mechanisms of ceramide-based responses to hypoxia.
  • To identify key enzymes and molecules involved in hypoxia-induced ceramide changes.
  • To investigate the role of dihydroceramides (DHCs) in cellular adaptation to low oxygen.

Main Methods:

  • Mass spectrometry-based survey of ceramide species under various hypoxic conditions (0.2-5% O2).
  • Analysis of DHCs in mammalian cells and rat lungs.
  • Gene silencing (siRNA) and overexpression of DHC desaturase (DEGS1/2).
  • Assessment of cell proliferation under different oxygen levels and DEGS activity.

Main Results:

  • A rapid, time-dependent, and proportional increase in DHCs was observed in response to hypoxia.
  • DHC accumulation occurred independently of hypoxia-inducible factors and was catalyzed by DEGS.
  • DEGS1/2 knockdown mimicked hypoxia's effects on DHCs and cell proliferation.
  • DEGS1/2 overexpression attenuated DHC accumulation and enhanced cell proliferation during hypoxia.

Conclusions:

  • Dihydroceramides (DHCs) are rapidly upregulated by hypoxia in a DEGS-dependent manner.
  • The DHC-based response to hypoxia functions independently of hypoxia-inducible factors.
  • Enzymatic desaturation of DHCs acts as an oxygen sensor, regulating ceramide metabolism and cell proliferation under physiological hypoxia.