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Updated: May 29, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

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Draft genome sequence of Lactobacillus malefermentans KCTC 3548.

Dong-Wook Kim¹, Sang-Haeng Choi, Aram Kang

¹Genome Resource Center, Korea Research Institute of Bioscience and Biotechnology, 111 Gwahangno, Yuseong-gu, Daejeon 305-806, Republic of Korea.

Journal of Bacteriology

|September 15, 2011

Summary

We present the draft genome sequence of Lactobacillus malefermentans KCTC 3548, a common lactic acid bacterium in beer production. This foundational genomic data offers insights into its role in brewing processes.

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Area of Science:

Microbiology
Genomics
Food Science

Background:

Lactic acid bacteria (LAB) are crucial in fermented food production, including beer.
Lactobacillus malefermentans is frequently identified during beer manufacturing.

Purpose of the Study:

To provide the draft genome sequence of Lactobacillus malefermentans KCTC 3548.
To establish a genomic resource for understanding LAB in brewing.

Main Methods:

Whole genome sequencing of Lactobacillus malefermentans KCTC 3548.
Genome assembly using Newbler Assembler 2.3.

Main Results:

The draft genome sequence size is 2,003,922 bp with a G+C content of 41.1%.
The genome was assembled into 172 large contigs (>100 bp).

Conclusions:

The availability of this genome sequence facilitates further research into Lactobacillus malefermentans.
This genomic data can enhance our understanding of LAB's contribution to beer flavor and stability.