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Updated: May 29, 2026

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
Published on: April 1, 2016
Electrochemical oxidation of glucose using mutant glucose oxidase from directed protein evolution for biosensor and
Eileen Hao Yu1, Radivoje Prodanovic, Güray Güven
1School of Chemical Engineering and Advanced Materials, Merz Court, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK. eileen.yu@ncl.ac.uk
Abstract:
In this study, electrochemical characterisation of glucose oxidation has been carried out in solution and using enzyme polymer electrodes prepared by mutant glucose oxidase (B11-GOx) obtained from directed protein evolution and wild-type enzymes. Higher glucose oxidation currents were obtained from B11-GOx both in solution and polymer electrodes compared to wt-GOx. This demonstrates an improved electrocatalytic activity towards electrochemical oxidation of glucose from the mutant enzyme. The enzyme electrode with B11-GOx also showed a faster electron transfer indicating a better electronic interaction with the polymer mediator. These encouraging results have shown a promising application of enzymes developed by directed evolution tailored for the applications of biosensors and biofuel cells.
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