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Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Mirabilis jalapa mottle virus: a new carlavirus infecting four o'clocks
Gregory J Hatlestad1, Lee Elam, Antonio Gonzalez
1Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712, USA.
Abstract:
Analysis of a next-generation sequence dataset from Mirabilis jalapa resulted in the discovery of a novel virus in the genus Carlavirus (family Betaflexiviridae), mirabilis jalapa mottle virus (MjMV). The complete genome of MjMV was determined to consist of 8315 nucleotides (nt), with the six open reading frames indicative of carlaviruses. MjMV is most similar to kalanchoe latent virus (60% identity) and lily symptomless virus (59% identity). The virus can be transmitted mechanically to Mirabilis, but thus far MjMV has only been shown to infect Mirabilis jalapa, causing a slight leaf mottling and leaf wrinkling phenotype.
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