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Updated: May 29, 2026

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
Published on: May 13, 2017
Spectroscopy and single-molecule emission of a fluorene-terthiophene oligomer
G E Khalil1, A M Adawi, B Robinson
1Department of Physics and Astronomy, University of Sheffield, Hicks Building, Hounsfield Road, Sheffield S3 7RH, United Kingdom.
Abstract:
We study the thiophene-based oligomer poly[2,7-(9,9-bis(2'-ethylhexyl)fluorene)-alt-2,5-terthiophene] (PF3T) in solution and when dispersed at low concentration into a polynorbornene matrix. We find that at high concentration in solution the 0-0 electronic transition observed in fluorescence is suppressed, a result indicative of the formation of weakly coupled H-aggregates. At low concentration in a polymer matrix, emission from both single molecules and molecular aggregates is observed. We find that the fluorescence spectra of most PF3T emitters are composed of a number of relatively narrow emission features, indicating that the emission usually occurs from multiple chromophores. A small number of PF3T molecules are however characterized by single chromophore emission, spectral blinking, and narrowed emission peaks.
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