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Related Experiment Video

Updated: May 29, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Single-shot, simultaneous incoherent and holographic microscopy.

E Shaffer¹, N Pavillon, C Depeursinge

¹École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland. etienne.shaffer@epfl.ch

Journal of Microscopy

|September 16, 2011

Summary

This study presents a new method for simultaneously capturing incoherent and holographic images in a single shot. This technique enables advanced microscopy applications by effectively separating different image signals.

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Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

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Published on: February 8, 2014

Area of Science:

Optics and Photonics
Microscopy
Digital Holography

Background:

Simultaneous acquisition of multiple image modalities is challenging.
Digital holographic microscopy offers high-resolution imaging but often requires separate recordings for different signal types.
Integrating incoherent and holographic imaging enhances microscopy capabilities.

Purpose of the Study:

To demonstrate a single-shot method for simultaneous recording of incoherent and holographic (intensity and phase) images.
To enable demultiplexing of these signals within the same camera frame.
To showcase applications in combined microscopy techniques.

Main Methods:

Utilizing carrier frequency modulation for signal separation in the spatial frequency domain.
Leveraging spatial oversampling inherent in off-axis digital holographic configurations.
Implementing a single-camera acquisition system.

Main Results:

Successful single-shot recording of one incoherent and two holographic images.
Effective demultiplexing of signals using the proposed spatial frequency domain approach.
Demonstrated applicability to combined fluorescence and digital holographic microscopy.
Demonstrated applicability to combined bright field and holographic second harmonic generation microscopy.

Conclusions:

The developed technique allows for efficient, simultaneous capture of diverse image types.
This method significantly advances combined imaging modalities in microscopy.
The approach is versatile and applicable to various advanced microscopy techniques.