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Related Experiment Video

Updated: May 29, 2026

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

Published on: August 9, 2017

Advances in multiphoton microscopy for imaging embryos.

Willy Supatto¹, Thai V Truong, Delphine Débarre

¹Laboratory for Optics and Bioscience, Ecole Polytechnique ParisTech, CNRS, and INSERM U696, Palaiseau, France. willy.supatto@polytechnique.edu

Current Opinion in Genetics & Development

|September 16, 2011

Summary

This summary is machine-generated.

Multiphoton imaging advances offer new ways to study embryonic development. This review clarifies microscopy techniques for live embryo imaging, covering speed, depth, and photodamage.

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Area of Science:

Developmental Biology
Microscopy
Systems Biology

Background:

Multiphoton imaging addresses challenges in systems biology and high-content analysis of embryonic development.
Recent advancements in multiphoton microscopy offer new possibilities for live embryo imaging.
Lack of clarity exists regarding the optimal microscopy technique for specific experimental objectives in embryo imaging.

Purpose of the Study:

To review and discuss key concepts in live embryo imaging.
To clarify the suitability of various multiphoton microscopy techniques for specific experimental goals.
To highlight recent applications and potentials in developmental biology.

Main Methods:

Discussion of key concepts: imaging speed, imaging depth, photodamage, and nonlinear contrast mechanisms.
Review of recent advances in multiphoton microscopy (light-sheet, laser scanning, adaptive, label-free).
Analysis of applications in live embryo imaging.

Main Results:

Recent advances enhance opportunities for live embryo imaging.
Understanding imaging speed, depth, photodamage, and contrast is crucial.
Various techniques offer distinct advantages for developmental biology studies.

Conclusions:

Multiphoton microscopy is vital for advancing systems biology and developmental studies.
Selecting the appropriate microscopy technique is key to successful live embryo imaging.
This review provides guidance for researchers in developmental biology.