Related Experiment Video
Updated: May 29, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
High yield production of D-xylonic acid from D-xylose using engineered Escherichia coli
Huaiwei Liu1, Kris Niño G Valdehuesa, Grace M Nisola
1Energy and Environment Fusion Technology Center (E2FTC), Myongji University, Yongin City, Gyeonggi Province, South Korea.
Abstract:
An engineered Escherichia coli was constructed to produce D-xylonic acid, one of the top 30 high-value chemicals identified by US Department of Energy. The native pathway for D-xylose catabolism in E. coli W3110 was blocked by disrupting xylose isomerase (XI) and xylulose kinase (XK) genes. The native pathway for xylonic acid catabolism was also blocked by disrupting two genes both encoding xylonic acid dehydratase (yagE and yjhG). Through the introduction of a D-xylose dehydrogenase from Caulobacter crescentus, a D-xylonic acid producing E. coli was constructed. The recombinant E. coli produced up to 39.2 g L(-1) D-xylonic acid from 40 g L(-1) D-xylose in M9 minimal medium. The average productivity was as high as 1.09 g L(-1) h(-1) and no gluconic acid byproduct was produced. These results suggest that the engineered E. coli has a promising application for the industrial-scale production of D-xylonic acid.
Related Concept Videos
Bioreactor Controls-III
Production of Pharmaceuticals
Production of Alcohol
Production of Organic Acids
Production of Antibiotics
