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Updated: May 29, 2026

Imaging of Extracellular Vesicles by Atomic Force Microscopy
Published on: September 11, 2019
Combination of transmission electron and atomic force microscopy techniques to determine volume equivalent diameter
Laarnie Tumolva1, Ji-Yeon Park, Kihong Park
1School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, Korea.
Abstract:
Morphological properties of atmospheric particles are directly related to their residence time and transport behaviors, and their deposition patterns in human respiratory systems. The projected properties of particles measured by transmission electron microscopy (TEM) were combined with the particle height measured by atomic force microscopy (AFM) to determine volume equivalent diameter of submicrometer particles. For nonvolatile (refractory) laboratory-generated spherical polystyrene latex and cubic NaCl particles, the measured volume equivalent diameters agreed well with the true values (within 4%). However, for nonrefractory (NH(4))(2)SO(4) particles, the measured volume equivalent diameter was much smaller than the true value due to evaporation of volatile species at low vacuum pressure and high electron-beam intensity conditions in TEM, and deformation of particles in AFM. We observed that the volume equivalent diameter of 100 nm mobility-classified atmospheric particles was 35 ± 5 nm, suggesting that these particles contain nonrefractory species, whereas that of 20 nm mobility-classified atmospheric particles was found to be 19 ± 6 nm, suggesting that these particles were refractory and spherical.
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