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Related Concept Videos

The Nucleosome01:19

The Nucleosome

Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
The Nucleosome02:33

The Nucleosome

DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside the nucleus is astonishing. It is organized into several sequentially higher levels of compaction to fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
The Nucleosome02:33

The Nucleosome

DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside the nucleus is astonishing. It is organized into several sequentially higher levels of compaction to fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
The Nucleosome Core Particle01:12

The Nucleosome Core Particle

Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
The Nucleosome Core Particle02:10

The Nucleosome Core Particle

Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
Nucleosome Remodeling02:54

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...

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Updated: May 29, 2026

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
10:40

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 10, 2013

Nucleosome positioning based on the sequence word composition.

Xian-Fu Yi1, Zhi-Song He, Kuo-Chen Chou

  • 1State Key Laboratory of Medical Genomics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Protein and Peptide Letters
|September 17, 2011
PubMed
Summary
This summary is machine-generated.

DNA sequence significantly influences nucleosome positioning in eukaryotes. This study identifies key sequence features, aiding understanding of genomic control mechanisms and DNA packaging.

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Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
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Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 10, 2013

In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy
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Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
06:32

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

Published on: March 9, 2022

Area of Science:

  • Molecular Biology
  • Genomics
  • Biophysics

Background:

  • Eukaryotic DNA is packaged into nucleosomes, fundamental units of chromatin.
  • Nucleosome positioning impacts DNA accessibility and all DNA-dependent processes.
  • Understanding nucleosome positioning is crucial for deciphering genomic control mechanisms.

Purpose of the Study:

  • To identify critical DNA sequence features governing nucleosome positioning.
  • To determine factors that favor or inhibit nucleosome formation.
  • To elucidate the role of DNA sequence in nucleosome organization in vivo.

Main Methods:

  • Utilized minimum redundancy maximum relevance (mRMR) for feature selection.
  • Employed the nearest neighbor algorithm (NNA) with incremental feature selection (IFS).
  • Analyzed 53,021 nucleosome and 50,299 linker DNA sequences from Saccharomyces cerevisiae.

Main Results:

  • Identified 32 significant sequence features from an initial set of 5,460.
  • Achieved an overall prediction accuracy of 76.5% via jackknife cross-validation.
  • Demonstrated that sequence-dependent DNA flexibility is key for nucleosome core particle positioning.

Conclusions:

  • DNA sequence plays a dominant role in nucleosome positioning.
  • Genome sequences facilitate rapid nucleosome reassembly rather than depletion.
  • Additional sequence features contribute to discriminating nucleosome-forming and inhibiting sequences.