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Basic fibroblast growth factor (bFGF) immunoreactivity is present in chromaffin granules.
R Westermann1, M Johannsen, K Unsicker
1Department of Anatomy and Cell Biology, University of Marburg, F.R.G.
Journal of Neurochemistry
|July 1, 1990
Summary
Basic fibroblast growth factor (bFGF) is found in bovine adrenal glands, specifically within secretory granules of medullary chromaffin cells. This growth factor exhibits neurotrophic activity, indicating its functional role in neuronal development.
Area of Science:
- Endocrinology
- Neuroscience
- Molecular Biology
Background:
- Basic fibroblast growth factor (bFGF) is a key signaling molecule involved in cell growth and differentiation.
- The presence and function of bFGF in the adrenal gland, particularly in secretory organelles, remain largely uncharacterized.
Purpose of the Study:
- To investigate the localization and forms of bFGF within bovine adrenal glands.
- To assess the neurotrophic activity of bFGF isolated from adrenal tissues.
Main Methods:
- Immunohistochemistry and immuno-electronmicroscopy were used to determine bFGF localization.
- Western blot analysis identified different molecular weight forms of bFGF.
- Neurotrophic activity was assessed using chick ciliary ganglion neurons.
Main Results:
- bFGF was detected in both the adrenal cortex and medulla, with specific localization in secretory granules of medullary chromaffin cells.
- Western blots revealed the typical 18-kDa bFGF and higher molecular weight forms (24, 30, 46 kDa).
- Adrenal bFGF fractions demonstrated significant neurotrophic activity, which was neutralized by antibodies.
Conclusions:
- This study provides the first evidence for bFGF localization within a cellular secretory organelle in the adrenal gland.
- The findings suggest the existence of novel, higher molecular weight immunoreactive forms of bFGF.
- Adrenal bFGF possesses functional neurotrophic properties, highlighting its potential role in neuroendocrine regulation.