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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: May 29, 2026

Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus

Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus

Published on: May 31, 2022

A proteomic approach to neuropeptide function elucidation.

L Temmerman¹, A Bogaerts, E Meelkop

¹Functional Genomics and Proteomics, Naamsestraat 59, Leuven, Belgium.

|September 17, 2011

Summary

This summary is machine-generated.

Neuropeptide PDF-1 knockout in C. elegans worms reveals new functions. Proteomics identified altered proteins in fat metabolism, stress resistance, and development, suggesting conserved roles similar to its vertebrate counterpart, VIP.

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Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

Published on: November 4, 2021

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Last Updated: May 29, 2026

Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus

Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus

Published on: May 31, 2022

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

Published on: November 4, 2021

Area of Science:

Neuroscience
Molecular Biology
Genetics

Background:

Neuropeptide functions are often difficult to determine due to their complex signaling.
Neuropeptide PDF-1 in C. elegans is known to affect locomotion.
The vertebrate homolog of PDF-1, vasoactive intestinal peptide (VIP), has diverse known functions.

Purpose of the Study:

To investigate novel functions of the neuropeptide PDF-1 in C. elegans.
To identify molecular changes associated with PDF-1 signaling.
To explore potential conserved functions between PDF-1 and VIP.

Main Methods:

Gel-based proteomics was employed.
A knockout model of the pdf-1 gene in C. elegans was utilized.
Protein levels were analyzed in pdf-1 null mutants compared to wild-type.

Main Results:

PDF-1 knockout altered protein levels involved in fat metabolism.
Knockout of PDF-1 affected proteins related to stress resistance.
Changes in protein levels associated with development were observed in pdf-1 mutants.

Conclusions:

Neuropeptide PDF-1 influences fat metabolism, stress resistance, and development in C. elegans.
These findings suggest conserved VIP-like functions for PDF-1.
Proteomics is a valuable tool for uncovering neuropeptide functions.