Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cloning of embryos.

R S Prather1, N L First

  • 1Department of Meat and Animal Science, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706.

Journal of Reproduction and Fertility. Supplement
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Knockout of SIGLEC1 in pigs reduces porcine reproductive and respiratory syndrome-1 (PRRSV-1) virus infection in primary macrophage cultures, but not in pigs.

Virology journal·2026
Same author

Effect of FGF2, LIF, IGF1 supplementation on pregnancy success following embryo transfer of in vitro derived embryos.

Theriogenology·2025
Same author

79 Cytokine supplementation to improve developmental competence of bovine embryos following slow-rate freezing.

Reproduction, fertility, and development·2022
Same author

Effects of RAD51-stimulatory compound 1 (RS-1) and its vehicle, DMSO, on pig embryo culture.

Reproductive toxicology (Elmsford, N.Y.)·2021
Same author

A novel swine sex-linked marker and its application across different mammalian species.

Transgenic research·2020
Same author

Emerging applications of sperm, embryo and somatic cell cryopreservation in maintenance, relocation and rederivation of swine genetics.

Theriogenology·2012
Same journal

Proceedings of the Seventh International Symposium on Equine Reproduction, July 12-17, 1998, Pretoria, South Africa.

Journal of reproduction and fertility. Supplement·2011
Same journal

Equine reproduction VII. Summing up.

Journal of reproduction and fertility. Supplement·2010
Same journal

Effect of transport on the intensity of stress reactions in mares and foals.

Journal of reproduction and fertility. Supplement·2010
Same journal

Gastrointestinal and endocrine function during 'foal heat diarrhoea' in healthy foals.

Journal of reproduction and fertility. Supplement·2010
Same journal

Response of newborn foals with thyroid musculoskeletal disease to adrenocorticotrophic hormone (ACTH).

Journal of reproduction and fertility. Supplement·2010
Same journal

Maternal influence on pre- and postnatal growth of foals born after embryo transfer.

Journal of reproduction and fertility. Supplement·2010
See all related articles

Nuclear transfer, a technique used since the 1950s, has been successfully applied to mammals like sheep and cattle. This method involves genomic reprogramming and can theoretically produce unlimited identical offspring.

Area of Science:

  • Developmental Biology
  • Reproductive Technologies
  • Genetics

Background:

  • Nuclear transfer has been a key technique for studying amphibian differentiation since the 1950s.
  • Recent advancements have enabled its application to mammalian embryos, though with species-specific limitations.

Purpose of the Study:

  • To explore the successful application of nuclear transfer in mammalian species.
  • To discuss the factors influencing nuclear reprogramming and its relation to embryo technologies.
  • To compare nuclear transfer with cloning by splitting.

Main Methods:

  • Application of nuclear transfer procedures developed for amphibians to mammalian embryos.
  • Analysis of species-specific timing of zygotic genome activation.
  • Consideration of micromanipulation and nuclear reprogramming.

Related Experiment Videos

Main Results:

  • Nuclear transfer is successful in sheep, cattle, rabbit, and pig embryos, but not in mouse embryos.
  • Species-specific timing of zygotic genome activation is a key factor in success.
  • Early embryos can serve as a source for unlimited identical offspring via serial nuclear transfer.

Conclusions:

  • Nuclear transfer requires genomic reprogramming of the donor nucleus.
  • The extent of differentiation that can be reprogrammed remains an area for further investigation.
  • Nuclear transfer offers a theoretical advantage for producing unlimited identical offspring compared to cloning by splitting.