Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
Karyotyping01:17

Karyotyping

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modeling the length distribution of gene conversion tracts in humans from the UK Biobank sequence data.

PLoS genetics·2025
Same author

Multiple-testing corrections in selection scans using identity-by-descent segments.

American journal of human genetics·2025
Same author

Estimating gene conversion rates from population data using multi-individual identity by descent.

American journal of human genetics·2025
Same author

Identity-By-Descent Mapping Using Multi-Individual IBD With Genome-Wide Multiple Testing Adjustment.

Genetic epidemiology·2025
Same author

Fast simulation of identity-by-descent segments.

Bulletin of mathematical biology·2025
Same author

Estimating gene conversion rates from population data using multi-individual identity by descent.

bioRxiv : the preprint server for biology·2025
Same journal

Genetic origins and constraints of evolutionary innovation.

Nature reviews. Genetics·2026
Same journal

Single-cell four-omics with CHARM.

Nature reviews. Genetics·2026
Same journal

Molecular integration of seasonal temperature signals in flowering time control.

Nature reviews. Genetics·2026
Same journal

RBPscan measures protein-RNA interactions in living cells.

Nature reviews. Genetics·2026
Same journal

Revisiting retinal and macular degeneration in the genomics era.

Nature reviews. Genetics·2026
Same journal

How evolution builds three morphs from one genome.

Nature reviews. Genetics·2026
See all related articles

Related Experiment Video

Updated: May 29, 2026

Pyrosequencing: A Simple Method for Accurate Genotyping
13:06

Pyrosequencing: A Simple Method for Accurate Genotyping

Published on: January 8, 2008

Haplotype phasing: existing methods and new developments.

Sharon R Browning1, Brian L Browning

  • 1Department of Biostatistics, University of Washington, Seattle, Washington 98195, USA. sguy@uw.edu

Nature Reviews. Genetics
|September 17, 2011
PubMed
Summary
This summary is machine-generated.

Determining haplotype phase is crucial for large-scale sequencing applications like variant imputation and understanding genetic disease links. This study reviews computational and statistical phasing methods, including emerging identity-by-descent techniques.

More Related Videos

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
08:48

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

Published on: June 28, 2012

A Novel Bayesian Change-point Algorithm for Genome-wide Analysis of Diverse ChIPseq Data Types
12:39

A Novel Bayesian Change-point Algorithm for Genome-wide Analysis of Diverse ChIPseq Data Types

Published on: December 10, 2012

Related Experiment Videos

Last Updated: May 29, 2026

Pyrosequencing: A Simple Method for Accurate Genotyping
13:06

Pyrosequencing: A Simple Method for Accurate Genotyping

Published on: January 8, 2008

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
08:48

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

Published on: June 28, 2012

A Novel Bayesian Change-point Algorithm for Genome-wide Analysis of Diverse ChIPseq Data Types
12:39

A Novel Bayesian Change-point Algorithm for Genome-wide Analysis of Diverse ChIPseq Data Types

Published on: December 10, 2012

Area of Science:

  • Genetics
  • Bioinformatics
  • Computational Biology

Background:

  • Haplotype phase determination is vital for large-scale sequencing.
  • Applications include variant imputation and disease susceptibility studies.
  • Methods include experimental and computational approaches.

Purpose of the Study:

  • To assess available haplotype phasing methods.
  • To focus on statistical and computational approaches.
  • To discuss practical application aspects and recent developments.

Main Methods:

  • Review of existing haplotype phasing techniques.
  • Focus on statistical and computational algorithms.
  • Exploration of identity-by-descent for phasing.

Main Results:

  • Comprehensive assessment of current phasing methodologies.
  • Discussion of practical considerations for method application.
  • Highlighting of novel approaches like identity-by-descent.

Conclusions:

  • Haplotype phasing is essential for modern genetic research.
  • Computational methods, especially statistical ones, are key.
  • Identity-by-descent shows promise for future phasing advancements.