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Related Experiment Video

Updated: May 29, 2026

Self-Assembly of Microtubule Tactoids

Self-Assembly of Microtubule Tactoids

Published on: June 23, 2022

Pulling it together: The mitotic function of TACC3.

Fiona E Hood¹, Stephen J Royle

¹The Physiological Laboratory; University of Liverpool; Liverpool, UK.

Bioarchitecture

|September 17, 2011

Summary

This summary is machine-generated.

Transforming acidic coiled coil 3 (TACC3) is crucial for mitotic spindle stability. New findings reveal TACC3, with ch-TOG and clathrin, forms cross-bridges stabilizing microtubules during cell division.

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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

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Published on: December 5, 2017

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Last Updated: May 29, 2026

Self-Assembly of Microtubule Tactoids

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Published on: June 23, 2022

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Transforming acidic coiled coil 3 (TACC3) is a non-motor microtubule-associated protein (MAP) essential for mitotic spindle stability and organization.
The precise mechanism of TACC3's microtubule-stabilizing function in the spindle has remained largely undefined.

Purpose of the Study:

To elucidate the molecular mechanism of TACC3 in stabilizing the mitotic spindle.
To present a comprehensive model integrating recent findings on TACC3 function.

Main Methods:

Review of recent studies identifying TACC3 complex composition and interactions.
Analysis of TACC3 phosphorylation and its role in complex formation.
Investigation of TACC3's function in stabilizing kinetochore fibers.

Main Results:

The TACC3 complex at microtubules includes clathrin and colonic and hepatic tumor overexpressed gene (ch-TOG).
Phosphorylated TACC3 directly interacts with ch-TOG and clathrin heavy chain, promoting their accumulation at the mitotic spindle.
The TACC3 complex stabilizes kinetochore fibers by forming cross-bridges between adjacent microtubules.

Conclusions:

TACC3 acts as an adaptor protein, recruiting ch-TOG and clathrin to mitotic microtubules in a manner regulated by Aurora A kinase.
This mechanism is critical for maintaining mitotic spindle integrity and proper chromosome segregation.