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Related Experiment Video

Updated: May 29, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

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Published on: May 17, 2016

Skeletal myogenesis and Myf5 activation.

Tanja Francetic¹, Qiao Li

¹Department of Cellular and Molecular Medicine; Faculty of Medicine; University of Ottawa; Ottawa, ON Canada.

|September 17, 2011

Summary

Myf5, a key regulator of muscle development, is controlled by intricate enhancers. Its expression is crucial for defining muscle types and also occurs in non-muscle cells like preadipocytes and neurons.

Area of Science:

Molecular Biology
Developmental Biology
Genetics

Background:

Myogenic regulatory factors (MRFs) are essential for skeletal muscle formation.
Myf5 is the earliest expressed MRF, with its regulation involving complex enhancers.
Myf5 expression is observed not only in developing muscles but also in preadipocytes and neurons.

Purpose of the Study:

To provide an updated overview of skeletal myogenesis regulation.
To highlight the intricate transcriptional activation of Myf5.
To discuss the diverse expression patterns of Myf5 in various cell types.

Main Methods:

Review of existing literature on myogenic regulatory factors.
Analysis of transcriptional regulation mechanisms.

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Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Published on: April 29, 2011

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Last Updated: May 29, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

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Published on: May 17, 2016

Identification and Analysis of Myogenic Progenitors In Vivo During Acute Skeletal Muscle Injury by High-Dimensional Single-Cell Mass Cytometry

Identification and Analysis of Myogenic Progenitors In Vivo During Acute Skeletal Muscle Injury by High-Dimensional Single-Cell Mass Cytometry

Published on: December 1, 2023

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Published on: April 29, 2011

Comparative study of Myf5 expression across different tissues.

Main Results:

Myf5 plays a pivotal role in determining muscle populations within the somite.
The transcriptional activation of Myf5 is highly complex, involving multiple regulatory elements.
Myf5 exhibits expression in distinct populations of myoblasts, preadipocytes, and neuronal tissues.

Conclusions:

Myf5 is a master regulator of skeletal myogenesis with complex transcriptional control.
Understanding Myf5 regulation is key to comprehending muscle development and its broader roles.
Myf5's expression in non-muscle tissues suggests additional functions beyond myogenesis.