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An Injectable and Drug-loaded Supramolecular Hydrogel for Local Catheter Injection into the Pig Heart
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DNA/protamine complex paste for an injectable dental material.

Tadao Fukushima1, Jun Ohno, Rieko Imayoshi

  • 1Center for Regenerative Medicine, Fukuoka Dental College, Sawara-ku, Fukuoka, Japan. tadaof@college.fdcnet.ac.jp

Journal of Materials Science. Materials in Medicine
|September 17, 2011
PubMed
Summary

Researchers developed a new injectable DNA/protamine complex paste for dental therapy. This biodegradable biomaterial shows potential for tissue repair and guided bone regeneration (GBR) or guided tissue regeneration (GTR) treatments.

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Area of Science:

  • Biomaterials Science
  • Dental Materials
  • Tissue Engineering

Background:

  • Injectable biomaterials are crucial for minimally invasive dental therapies.
  • Current materials often lack ideal properties for defect repair and regeneration.
  • DNA/protamine complexes offer potential for novel biomaterial development.

Purpose of the Study:

  • To develop and characterize a new injectable DNA/protamine complex paste for dental applications.
  • To evaluate the physical properties, biocompatibility, and degradation of the DNA/protamine paste.
  • To assess the preliminary antibacterial activity and potential for tissue regeneration.

Main Methods:

  • DNA and protamine sulfate were reacted to form a complex powder.
  • The powder was kneaded with distilled water to create a paste.
  • Fourier-transform infrared (FT-IR) spectroscopy confirmed complex formation.
  • Viscosity, injectability through a fine needle, antibacterial effects, and in vivo tissue response were assessed.

Main Results:

  • A porous DNA/protamine complex paste with suitable viscosity (approx. 280.1 Pas) was successfully prepared.
  • The paste could be easily injected through a 0.25 mm internal diameter needle.
  • The paste exhibited limited, temporary delay in the growth of specific oral bacteria but did not kill them.
  • In vivo studies showed mild tissue response and gradual degradation of the implanted paste in rats.

Conclusions:

  • The DNA/protamine complex paste is a promising injectable and biodegradable biomaterial for dental applications.
  • Its properties suggest suitability for syringe-based delivery in procedures like tissue defect repair, GBR, and GTR.
  • Further research is warranted to enhance antibacterial efficacy and optimize regenerative potential.