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Updated: Aug 12, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Published on: June 6, 2017
Periodic biosynthesis of the human M-phase promoting factor catalytic subunit p34 during the cell cycle
C H McGowan1, P Russell, S I Reed
1Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Abstract:
The product of the CDC2Hs gene is the protein kinase subunit of the M-phase promoting factor, which is required for entry into mitosis. The activity of this kinase is regulated in a cell cycle-dependent manner by reversible phosphorylation and through association with other proteins. We report here that in HeLa cells, the abundance of the CDC2Hs mRNA and the rate of synthesis of the encoded protein, p34, vary in a cell cycle-dependent manner.
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