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Related Experiment Video

Updated: May 29, 2026

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells
09:41

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

Published on: July 15, 2015

Sample processing and methodological pitfalls in multiple myeloma research.

A Potácová1, J Stossová, I Buresová

  • 1Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

Klinicka Onkologie : Casopis Ceske a Slovenske Onkologicke Spolecnosti
|September 20, 2011
PubMed
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This study details methods for isolating plasma cells from biological samples, crucial for multiple myeloma research. Techniques like CD138 MicroBeads and cell sorting address challenges in multiple myeloma research.

Area of Science:

  • Hematology and Oncology
  • Molecular Biology
  • Biotechnology

Background:

  • Accurate isolation of plasma cells is critical for understanding and researching multiple myeloma.
  • Existing methods face challenges, particularly with low-abundance cell populations and sample heterogeneity.

Purpose of the Study:

  • To describe optimized procedures for biological material processing and cell separation for multiple myeloma research.
  • To detail the application of Magnetic-Activated Cell Sorting and Fluorescence-Activated Cell Sorting for plasma cell isolation.
  • To identify and discuss research pitfalls in multiple myeloma studies.

Main Methods:

  • Processing of biological material and implementation of cell separation algorithms.
  • Use of CD138 MicroBeads and Auto-Magnetic-Activated Cell Sorting for samples with > 5% plasma cells.

Related Experiment Videos

Last Updated: May 29, 2026

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells
09:41

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

Published on: July 15, 2015

  • Application of Fluorescence-Activated Cell Sorting for low-abundance plasma cell detection using fluorescently labeled antibodies.

    • Main Results:

    • Successful isolation of plasma cells suitable for downstream molecular biological, cytogenetic, and protein analyses.
    • Demonstrated efficacy of distinct cell sorting techniques based on plasma cell abundance.
    • Identified and addressed several research pitfalls encountered in multiple myeloma studies.

    Conclusions:

    • The described methods provide robust plasma cell isolation for comprehensive multiple myeloma research.
    • The study highlights advancements in cell separation techniques and acknowledges ongoing challenges in the field.