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Flow cytometric phenotyping and analysis of T regulatory cells in multiple myeloma patients

K R Muthu Raja1, L Kovárová, J Stossová

  • 1Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

Klinicka Onkologie : Casopis Ceske a Slovenske Onkologicke Spolecnosti
|September 20, 2011
PubMed

Multiple myeloma (MM) is a plasma cell (PC) disorder and associated with immune impairments. Flow cytometry based phenotyping and quantification of regulatory T cells (Tregs) enable to monitor the immune status of myeloma patients. Apart from enumeration of Tregs, functional characterization using proliferation or suppression assay adds key value in demonstrating the functional value ofTregs. Our study revealed that in MM patientsTregs are elevated compared to healthy subjects, which demonstrate the immune deregulation in MM.

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Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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