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Updated: Aug 3, 2026

Detection of Signaling Effector-Complexes Downstream of BMP4 Using in situ PLA, a Proximity Ligation Assay
Published on: March 4, 2011
[Detection of protease-sensitive factors with properties of beta-type transforming growth factor in early loach
Abstract:
The blastoderm and yolk of Misgurnus fossilis L. late blastulae were subjected to acid-ethanol extraction and gel filtration on biogel P10 in 1 M CH3COOH. This resulted in isolation of polypeptides that induce substrate-independent proliferation of NIH-3T3 mouse fibroblasts in presence of epidermal growth factor. They did not compete for epidermal growth factor receptors of A-431 cells and inhibited substrate-independent proliferation of human lung carcinoma A-549 cells. These properties of the growth factor-like polypeptides from loach embryos are similar to those of transforming growth factor beta.

