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Updated: May 29, 2026

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
Published on: May 27, 2022
Global population structure of Aspergillus terreus inferred by ISSR typing reveals geographical subclustering
Carolyn O S Neal1, Aaron O Richardson, Steven F Hurst
1Mycotic Diseases Branch, Center for Disease Control and Prevention, 1600 Clifton Rd NE, Atlanta, GA 30329, USA.
Background:
Aspergillus terreus causes invasive aspergillosis (IA) in immunocompromised individuals and can be the leading cause of IA in certain medical centers. We examined a large isolate collection (n = 117) for the presence of cryptic A. terreus species and employed a genome scanning method, Inter-Simple Sequence Repeat (ISSR) PCR to determine A. terreus population structure.
Results:
Comparative sequence analyses of the calmodulin locus revealed the presence of the recently recognized species A. alabamensis (n = 4) in this collection. Maximum parsimony, Neighbor joining, and Bayesian clustering of the ISSR data from the 113 sequence-confirmed A. terreus isolates demonstrated that one clade was composed exclusively of isolates from Europe and another clade was enriched for isolates from the US.
Conclusions:
This study provides evidence of a population structure linked to geographical origin in A. terreus.
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