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Related Experiment Video

Updated: May 29, 2026

Gene Trapping Using Gal4 in Zebrafish
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Gene Trapping Using Gal4 in Zebrafish

Published on: September 29, 2013

Generating conditional mutations in zebrafish using gene-trap mutagenesis.

Lisette A Maddison1, Jianjun Lu, Wenbiao Chen

  • 1Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Methods in Cell Biology
|September 20, 2011
PubMed
Summary
This summary is machine-generated.

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Researchers developed a new gene-editing strategy for zebrafish, enabling precise control over gene function in specific tissues or at particular times. This advance facilitates detailed studies of gene roles in development and disease.

Area of Science:

  • Genetics
  • Developmental Biology
  • Molecular Biology

Background:

  • Existing mutagenesis methods in zebrafish lack tissue- or temporal-specificity.
  • Understanding gene function in specific cellular contexts is crucial for biological research.

Purpose of the Study:

  • To develop a novel strategy for targeted gene disruption in zebrafish.
  • To enable tissue- or temporal-specific functional analysis of genes.

Main Methods:

  • Combined gene-trap mutagenesis with FlEx modules and site-specific recombinases (Cre and Flp).
  • Utilized a gene-trap cassette with orientation-dependent mutagenicity and fluorescent protein indicators.
  • Employed transposon delivery for efficient cassette integration.

Main Results:

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Last Updated: May 29, 2026

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  • Developed a versatile gene-trap system allowing for two rounds of stable inversion.
  • The system provides orientation-specific gene disruption with fluorescent readouts.
  • The strategy is amenable to large-scale, genome-wide application.

Conclusions:

  • This novel gene-trap strategy offers precise spatiotemporal control over gene function in zebrafish.
  • It overcomes limitations of previous mutagenesis techniques.
  • Facilitates in-depth investigation of gene function across various cell types and developmental stages.