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Related Experiment Video

Updated: May 29, 2026

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
11:42

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

Sequencing-based expression profiling in zebrafish.

Jin Liang, Gabriel Renaud, Shawn M Burgess

    Methods in Cell Biology
    |September 20, 2011
    PubMed
    Summary
    This summary is machine-generated.

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    This chapter details a Digital Gene Expression (DGE) protocol for zebrafish research, enabling molecular-level study of biological processes using next-generation sequencing. It covers experimental and data analysis steps for accurate gene expression profiling.

    Area of Science:

    • Molecular Biology
    • Genomics
    • Zebrafish Research

    Background:

    • Gene expression profiling is crucial for understanding molecular-level biological processes.
    • Next-generation sequencing has advanced high-throughput expression profiling.
    • Zebrafish are a key model organism in biological research.

    Purpose of the Study:

    • To describe a protocol for Digital Gene Expression (DGE) in zebrafish.
    • To provide guidelines for both wet-bench experiments and bioinformatics analysis.
    • To highlight potential challenges associated with DGE in this model system.

    Main Methods:

    • Detailed protocol for Digital Gene Expression (DGE) library preparation.
    • Guidelines for high-throughput sequencing of zebrafish samples.

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    Related Experiment Videos

    Last Updated: May 29, 2026

    Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
    11:42

    Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

    Published on: October 27, 2017

    Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
    13:14

    Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

    Published on: August 10, 2009

    Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
    10:17

    Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

    Published on: April 30, 2018

  • Bioinformatics pipeline for DGE data analysis, including quality control and differential expression analysis.

    • Main Results:

    • Successful application of DGE protocol in zebrafish research.
    • Comprehensive data analysis enabling identification of tissue/organ-specific gene expression patterns.
    • Identification of key considerations and potential pitfalls for DGE implementation.

    Conclusions:

    • The described DGE protocol is effective for zebrafish gene expression studies.
    • This method facilitates molecular insights into zebrafish biology.
    • Awareness of potential challenges ensures robust and reliable experimental outcomes.