Aquaculture and husbandry at the zebrafish international resource center

This chapter provides an overview of the aquaculture system and the husbandry methods used at the Zebrafish International Resource Center (ZIRC). ZIRC employs a recirculating water system with mechanical and biological filtration for its main facility, and a flow-through water system for its quarantine room. I describe basic husbandry methods for breeding, rearing larvae, and feeding and the extensive in-house health surveillance for the biosafety of the fish population. Whereas the description of these procedures is merely an overview and only the most essential methods are included, it offers several starting points to set up, refine, or troubleshoot other fish facilities.