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Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

When multiphoton microscopy sees near infrared.

Halina Mojzisova¹, Julien Vermot

¹IGBMC (Institut de Génétique et de Biologie Moléculaire et Cellulaire), Inserm U964, CNRS UMR7104, Université de Strasbourg, 1 rue Laurent Fries, Illkirch F-67404, France.

Current Opinion in Genetics & Development

|September 20, 2011

Summary

Multiphoton microscopy offers advanced developmental biology imaging by combining fluorescent imaging, laser ablation, and harmonic generation. New optical parametric oscillator (OPO) techniques enable deeper tissue visualization for real-time developmental process studies.

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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Area of Science:

Developmental Biology
Microscopy
Biophotonics

Background:

Sophisticated imaging is crucial for quantifying and visualizing developmental processes in real-time.
Multiphoton microscopy is a versatile technique integrating fluorescent imaging, laser ablation, and harmonic generation.

Purpose of the Study:

To review promising multiphoton microscopy approaches utilizing optical parametric oscillators (OPOs).
To highlight applications of nonlinear microscopy in developmental biology.
To discuss challenges in applying these techniques to embryo imaging.

Main Methods:

Multiphoton microscopy
Optical parametric oscillators (OPOs)
Nonlinear microscopy

Main Results:

Recent advances in femtosecond lasers and OPOs allow imaging at novel wavelengths within the tissue transparency window.
Multiphoton microscopy enables simultaneous fluorescent imaging, laser ablation, and harmonic generation.

Conclusions:

OPO-based multiphoton microscopy provides advanced capabilities for studying developmental biology.
Nonlinear microscopy offers significant potential for real-time visualization of embryonic development.
Experimental challenges exist but are being addressed for improved embryo imaging.