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Related Concept Videos

Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
Anaphase A and B01:39

Anaphase A and B

Microtubules form through the end-to-end polymerization of tubulin heterodimers. Kinetochore microtubules originate from the spindle poles, and their plus-ends connect with the kinetochores on sister-chromatids. Ndc80 protein complexes, present on the kinetochore, form low-affinity links with the plus end of these kinetochore microtubules.
Plus-end depolymerization releases tubulin heterodimers from the terminal region of the microtubule. As tubulin subunits are lost, the Ndc80 complexes detach...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Destabilization of Microtubules01:45

Destabilization of Microtubules

The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...

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Related Experiment Video

Updated: May 29, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

HURP regulates chromosome congression by modulating kinesin Kif18A function.

Fan Ye1, Lora Tan, Qiaoyun Yang

  • 1Department of Biological Sciences, National University of Singapore, Singapore 117543.

Current Biology : CB
|September 20, 2011
PubMed
Summary

Hepatoma upregulated protein (HURP) interacts with Kif18A to regulate chromosome alignment during cell division. Overexpressing HURP

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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Accurate chromosome segregation during mitosis depends on controlled microtubule dynamics.
  • Microtubule-associated proteins (MAPs) like HURP regulate kinetochore microtubule (K-MT) dynamics.
  • HURP localizes to k-fibers and stabilizes them, promoting chromosome congression.

Purpose of the Study:

  • To elucidate the molecular mechanism of HURP in regulating chromosome congression.
  • To investigate the interaction between HURP and Kif18A.
  • To understand HURP's role in Kif18A dynamics and chromosome movement.

Main Methods:

  • Overexpression of HURP N-terminal domain (HURP(278)) to induce mitotic defects.
  • Coimmunoprecipitation and bimolecular fluorescence complementation assays to detect protein interactions.
  • Live-cell imaging to analyze protein localization and dynamics.

Main Results:

  • HURP(278) overexpression mimics Kif18A depletion effects, causing mitotic defects.
  • Kif18A is identified as a novel interaction partner of HURP.
  • HURP regulates Kif18A localization and dynamics at K-MT plus ends, and Kif18A overexpression partially rescues HURP(278)-induced chromosome misalignment.

Conclusions:

  • HURP and Kif18A interact and cooperate to regulate chromosome congression.
  • This study reveals a novel regulatory mechanism for Kif18A during mitosis.
  • Findings provide new insights into chromosome movement at the metaphase plate.