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Using X-ray Crystallography, Biophysics, and Functional Assays to Determine the Mechanisms Governing T-cell Receptor Recognition of Cancer Antigens
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TLR expression in human melanoma cells.

Mélanie Saint-Jean1, Anne-Chantal Knol, Jean-Michel Nguyen

  • 1Immuno-dermatology laboratory, Onco-dermatology unit, CIC biothérapie INSERM 0503, CHU Hôtel-Dieu, 1 place A. Ricordeau, 44093 Nantes Cedex 1, France.

European Journal of Dermatology : EJD
|September 20, 2011
PubMed
Summary
This summary is machine-generated.

Melanoma cells express Toll-like receptors (TLRs) 7 and 8, with varying protein levels observed between cell lines and lymph nodes. This research expands understanding of TLR expression in melanoma.

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Experimental Metastasis Assay
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Published on: August 24, 2010

Area of Science:

  • Immunology
  • Oncology
  • Molecular Biology

Background:

  • Melanoma cells are known to express Toll-like receptors (TLRs) 2, 3, and 4.
  • The expression patterns of other TLRs in melanoma remain largely uncharacterized.

Purpose of the Study:

  • To investigate the expression of TLR2, -3, -4, -7, -8, and -9 in melanoma cell lines and melanoma-invaded lymph nodes.
  • To determine both mRNA and protein expression levels of these TLRs.

Main Methods:

  • Quantitative reverse transcription-polymerase chain reaction (RT-PCR) for mRNA analysis.
  • Ex vivo immunohistochemistry and in vitro flow cytometry for protein expression analysis.

Main Results:

  • Melanoma cells expressed TLR2, -3, -4, -7, and -9 mRNA in vitro and potentially ex vivo.
  • TLR2 and -4 protein expression was high ex vivo (>50%) but absent in vitro.
  • TLR-3 and -8 proteins showed low ex vivo expression with high in vitro expression.
  • TLR-7 and -9 proteins were expressed both ex vivo and in vitro.
  • This study is the first to demonstrate TLR7 and -8 expression in melanoma cells.

Conclusions:

  • Melanoma cells express a range of TLRs, including TLR7 and -8, which was previously unknown.
  • Differential expression of TLRs at mRNA and protein levels, and between in vitro and ex vivo settings, suggests complex regulatory mechanisms in melanoma.