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Rapid screening method for compounds that affect the growth and germination of Candida albicans, using a real-time

Lucja M Jarosz1, Bastiaan P Krom

  • 1Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, Groningen, The Netherlands.

Applied and Environmental Microbiology
|September 20, 2011
PubMed

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Real Time RT-PCR02:57

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...